Instrument Name:Sorting flow cytometry

Spectrometer System Specifications:BeckmanMoFloXDP

Manufacturer:Beckman Coulter

Applications:

Qualitative or quantitative detection and accurate separation of fluorescent particles. Mainly used in biology, targeted drug screening and pharmacology,etc.

Instrument main functions:

Cells in complex samples can be identified, classified, quantified and separated, it can purify one to four specific cellssimultaneouslyat a single time by ultrahigh-speed sorting, high-throughput monoclonal sorting or cell microarray preparation. Less than 5% of the sample cells are discarded to ensure a high recovery rate of target cells in the sample, especially suitable for research on extremely low content cells such as stem cells.

Technical specifications of the instrument:

1. Optical system:

Air excitation, no need to worry about fragment signals generated by quartz cup aging, maintaining stable performance; Loading three different wavelengths oflasers，namely488nm, 561nm and 355nm, which can detect 2 forward-lateralchannels and 9 fluorescence channels.u

2. Fully automatic closed liquid flow system:：

uThe liquid flow pressure is infinitely adjustable from 0 to 100 psi; Three-level filtration system, which effectively filters 99.999% of the impurities > 0.1μm in sheath solution; Teflon materialpipeline, resistant to acid, alkali, and high temperature, and can be washed with various volumes;Different sizes of nozzles to accurately adapt to any tissue derived cell; The Smart Sampler automatic sampling system, which can support different sizes of sample tube, and has functions such as fully automatic cleaning, backwashing, bubble removal, and temperature control.

3. Cell sorting system:

uThe theoretical maximum separation speed of 200000 cells per second; Separation purity >99%; Support 4-way sorting and different types of orifice sorting.

4. Electronic and signal processing system:

Provide automatic and real-time compensation.

5. Fully automatic integrated data management system.

Person in charge:

LiuQian,Dou Daolei, Zhao Xiangshuai

Tel:022-85358172

Email ：liuqian@nankai.edu.cn

doudaolei@nankai.edu.cn

reality.2006@163.com

Address:D124-1, Comprehensive Laboratory Building, Nankai University, Jinnan Campus