Instrument Name:Laser confocal scanning microscope

Spectrometer System Specifications:TCS SP8

Manufacturer:Leica

Applications:Fluorescence analysistoprovideresearchers with high-precision 3D imaging data, accurate subcellular structure and dynamic imaging

Instrument main functions:

Laser confocal scanning microscope applies laser as scanning light source for tomographic imaging of fluorescent samples, which can be used to observe cell morphology and obtain fluorescent images of microstructure inside cells or tissues; it can also be used for quantitative analysis of intracellular biochemical components, optical density statistics and cell morphometry, etc., to observe changes such as Ca2+, PH value, membrane potential, etc. at the subcellular level, and can be used with It can be used to observe changes such as Ca2+, PH, membrane potential, etc. at subcellular level, and can be combined with the focus stabilization system to achieve long time live cell dynamic observation and 3D imaging. It has become a new generation of powerful research tools in morphology, molecular biology, neuroscience, pharmacology, genetics and other fields.

Technical specifications of the instrument:

1,Laser: 405nm, argon ion (458nm, 476nm, 488nm, 514nm), 561nm, 594nm, 633nm.

2,High sensitivity detectorHyD

3,12kHz fast scanner

4,Eyepiece: 10×, field of view 25mm

5, Objective: 10×(dry, N.A.=0.40);20×(dry, N.A.=0.70); 40×(oil, N.A.=0.60); 63×(oil, N.A.=1.4-1.6)

6,Fast AOTF, 8 channels, switching time <5ms.

7,Live cell culture system.

8,Equipped with FRAP and FRET experiment module, 3D layer sweep and reconstruction function, calcium ion determination and analysis.

Person in charge:Xiao Nannan,Zhangyan

Tel:022-85358318

Email ：xiaonn@nankai.edu.cn

zhangyan2016@nankai.edu.cn

Address:D317, Comprehensive Laboratory Building, Nankai University, Jinan New Campus