Instrument Name:High-resolution wide-field fluorescence microscope

Spectrometer System Specifications:Tirf&Thunder

Manufacturer:Leica

Applications:Provides fast and convenient operation of wide-field microscopy while combining with the inverse convolution technology to achieve imaging resolution close to that of laser confocal microscopy, suitable for imaging and analysis of tissue sections, organoids, and living cells.

Instrument main functions:

Equipped with real-time high-definition three-dimensional imaging module (Thunder), combining optical and digital processing; automatic interpretation of objective parameters, simultaneous deconvolution processing, removal of stray light signals in the non-focal plane of the sample, to achieve high fluorescence resolution to 140nm in the XY direction, imaging depth of 500μm for non-transparent samples; equipped with total internal reflection fluorescence imaging module (TIRF), fully automatic one-touch automatic calibration, support TIRF different polarization angle incidence settings, accurate determination of the displayed and adjustable TIRF penetration depth, support fast, low phototoxicity of ultra-high resolution imaging

Technical specifications of the instrument:

1, Objective: 5× (dry, N.A.= 0.12); 10× (dry, N.A.= 0.32); 20× (dry, N.A.= 0.4) ; 40× (dry, N.A.= 0.95); 63× (oil, N.A.= 1.47)

2, Fluorescence filter:405: ex375-435nm/em450-490nm;GFP: ex450-490nm/em500-550nm; TXR:ex540-580nm/em593-667nm; Y5:ex590-650nm/em663-737nm

3, High sensitivity sCOMS camera, imaging resolution: 2048×2048.

4, Live cell culture system with autofocus, automatic real-time focus lock function, CO25.0%~20.0%, temperature control range: room temperature +3℃~60℃

5, Total internal reflection module (TIRF):contains 488nm laser, supports time series shooting, the imaging range of total internal reflection image is not less than 200μm×200μm under 63× objective lens.

6, Support Large-scale puzzles, compatible with plastic culture dishes and multi-well plates, common slides, chamber slides.7, Real-time image processing functions, including complete image processing, multi-dimensional image rendering, measurement, cell genealogy study, nerve (branch-like structure) analysis, cell and correlation analysis, etc.

Person in charge:Xiao Nannan,Zhangyan

Tel:022-85358318

Email ：xiaonn@nankai.edu.cn

zhangyan2016@nankai.edu.cn

Address:D317, Comprehensive Laboratory Building, Nankai University, Jinan New Campus