Instrument Name:Super-resolution fluorescence microscope

Spectrometer System Specifications:Elyra 7

Manufacturer:Zeiss

Applications:Compatible with conventional fluorescence microscopy samples, it breaks through the diffraction limits of conventional microscopy to discern ultra-fine subcellular organelle structures and to image live and fixed samples with ultra-high speed for low phototoxicity.

Instrument main functions:

Equipped with structured light illumination (Lattice SIM), the ultimate resolution of 120nm inxy-axis and 300nm in z-axis, to achieve fast, low phototoxicity and ultra-high resolution imaging of optical sections; equipped with single molecule localization microscopy (SMLM) system, compatible with PALM, STORM,dSTORMand other imaging technologies, the resolution reaches 20nm inxy-axis and 50nm in z-axis.

Technical specifications of the instrument:

1, Microscope part: inverted fluorescence microscope; live cell incubator; motorized XY-directional scanning stage; Z-axis piezoelectric loading stage.

2, Objective: 100×/1.57 oil (DIC); 63×/1.46 oil; 40×/1.2 water; 10×/0.3 dry.

3, Illumination and detection system: solid-state laser, including 405nm, 488nm, 561nm, 639nm; doublesCMOScamera.

4, Illumination mode: fully electric falling fluorescence (EPI), oblique illumination (HILO) and total internal reflection illumination (TIRF)

5, Perfect focus system: support jigsaw puzzle, compatible with plastic Petri dishes and multi-well plates, common slides, chamber slides.

6, Single molecule fluorescence localization microscope image real-time processing function, including complete image processing function, can calculate the fluorescence molecule position according to the intensity of the light spot.

Person in charge:Xiao Nannan,Zhangyan

Tel:022-85358318

Email ：xiaonn@nankai.edu.cn

zhangyan2016@nankai.edu.cn

Address:D317, Comprehensive Laboratory Building, Nankai University, Jinan New Campus