Instrument Name:Laser confocal scanning microscope

Spectrometer System Specifications:LSM 800

Manufacturer:Zeiss

Applications:Fluorescence analysistoprovideresearchers with high-precision 3D imaging data, accurate subcellular structure and dynamic imaging

Instrument main functions:

Laser confocal scanning microscope applies laser as scanning light source for tomographic imaging of fluorescent samples, which can be used to observe cell morphology and obtain fluorescent images of microstructure inside cells or tissues; it can also be used for quantitative analysis of intracellular biochemical components, optical density statistics and cell morphometry, etc., to observe changes such as Ca2+, PH value, membrane potential, etc. at the subcellular level, and can be used with It can be used to observe changes such as Ca2+, PH, membrane potential, etc. at subcellular level, and can be combined with the focus stabilization system to achieve long time live cell dynamic observation and 3D imaging. It has become a new generation of powerful research tools in morphology, molecular biology, neuroscience, pharmacology, genetics and other fields.

Technical specifications of the instrument:

1, All-solid-state laser: 405nm; 488nm; 561nm; 640nm.

2, Detector type and number: 1 GaAsP ultra-high sensitivity detector; 2 PMT detectors; 1 transmissive bright field and DIC detector.

3, Scanning optical zoom: zoom range 0.5 x - 40x, in steps of 0.1 x.

4, Objective:10x(dry, N.A.=0.45);20x(dry, N.A.=0.80); 40x(oil, N.A.=1.3); 63x(oil, N.A.≥1.4)

5, ultra-high resolution imaging module Airyscan, XY-axis resolution up to 120nm, Z-axis resolution up to 300nm.

6.Co-location analysis and spectral splitting and other data processing

Person in charge:Xiao Nannan,Zhangyan

Tel:022-85358318

Email ：xiaonn@nankai.edu.cn

zhangyan2016@nankai.edu.cn

Address:D317, Comprehensive Laboratory Building, Nankai University, Jinan New Campus